Effect of Nanoherbal Haramonting (Rhodomyrtus tomentosa) on Lungs Histology

The study aim to know to effect of Nanoherbal Rhodomyrtus tomentosa (Haramonting) on lungs histology of Mus musculus. Haramonting in Indonesia as a new source of health-promoting compounds such as dietary fibers, essential fatty acids, and phenolic compounds because there are 19 phenolic compounds including stilbenes and ellagitannins as major components, followed by anthocyanins, flavonols, and gallic acid. Haramonting is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The lungs were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). There were a significant difference (P<0,05) between control group and treatment groups on form of lung cells although no significant in lung weigh

Review Journals : Molecular From Anonaceae

There are roughly 120 species of the Anona genus, which is found in tropical and subtropical areas. The diversity at the species level and similarity of the leaves, fruits, and stems that are produced as a result of plant cultivation and hybridization create new plants that are difficult to macroscopically identify. The diversity of the genus Anona can be accurately determined using molecular identification. For example, genetic analysis utilizing PCR and the RAPD and AFLP procedures is one way to gather molecular data. In this study, we inform the development of species-specific primers by differentiating the most agronomically interesting Annona species that, until recently, a new formal classification based on molecular phylogenetics has been developed in order to have a quick and precise method of distinguishing Annona between species agronomically

The Effect Of Giving Nano Herbal Biwa Leaf (Eriobotrya japonica Lindl.) Against Histological Description Of Kidney Rat That Induced By Alloxan

Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to abnormal insulin secretion and insulin action. Uncontrolled diabetes mellitus will cause various organ complications, one of which is diabetic nephropathy or kidney failure. The aim of this study was to determine the effect of nano herbal of biwa leaves (Eriobotrya japonica) on the structure of gromerulus, renal necrosis, proximal tubular constriction, kidney weight and kidney discoloration. This study uses an analytic study with a true experimental design in male rats (Rattus norvegicus) consisting of five treatments with each of six replications. This study consisted of a negative control group that was only given ad-libitum feed, a positive control injected with alloxan, and two groups injected with alloxan and given a 250 mg/kg and 500 mg/kg biwa nanoherbal. It was concluded that the nanoherbal of biwa leaves is effective enough to reduce blood glucose levels and is safe for consumption by diabetics because it does not damage kidney cells

Immunohistochemistry examination to reveal the pathogenesis of Oral Squamous Cell Carcinoma

Oral mucosal cancer is a type of cancer that develops from the lining of the oral cavity (mucosa). The main risk factors are smoking and drinking alcohol. The pathogenesis of oral mucosal cancer involves various interrelated etiologies such as smoking and alcohol consumption, human papilloma virus (HPV), and patients who have undergone hemopoietic stem cell transplants (stem cell transplants). Meanwhile, it does not rule out the possibility of internal factors such as genetics. There are several types of oral mucosal cancer, but oral squamous cell carcinoma is the most common type of oral cancer and represents more than 90% of all head and neck cancers. Immunohistochemical examination of the Oral squamous cell carcinoma smear biopsy material which included examination of antibodies in the form of cytokeratin, CDT1, Ki-67, Geminin, Mcm2, γ-H2A/ histone family member X and Aurora-A, BCL-2, VEGF, p53, p63, p,73, Prb, c-erbB2 or HER2/neu, upregulation of telomerase (human telomerase reverse transcriptase; hTERT), loss of heterozygosity (Chromosome loci 3p, 8p, 9p, 4q, 11q, 13q, 17p), High-risk Human papillomavirus 16/18 (HR-HPV16 and 18), p16, Overexpression of EGFR, c-jun, c-fos, surviving (BIRC5), MMP-9, MMP9, TGF-, COX-1, and-2, and amplification of Cyclins D and E. Thus, paving the way for appropriate therapy

Stem Cells: DNA Microarray Approach

In recent years, stem cell therapy has become a very promising and advanced scientific research topic. The development of treatment methods has evoked great expectations, not only researchers but also society due to its potency in cell-based therapy. Stem cells are unspecialized cells of the human body. They are able to differentiate into any cell of an organism and have the ability of self-renewal. Broadly speaking, the application of stem cells can be divided into two, namely: autotransplantation (donor and recipient are same person) and allotransplantation (donors and recipients are people who different). However, other applications namely xenotransplantation (donor and the recipient is the species that different) now it also becomes center of attention. Since it is challenging to isolate stem cells, molecular methods specifically the use of a DNA microarray, are one way to find stem cells. Microarrays have been applied in stem cell research to identify major features or expression signatures that define stem cells and characterize their differentiation programs toward specific lineages. This paper is a review focused on a discussion of the of the microarray technology and types of stem cell transplant

Does Chitala Lopis Really Extinct? An Overview And DNA Barcode (COI) Comparison

The Asian Featherback Fish Or Ikan Belida Is One Of The Most Important Species Of Fish In Indonesia That Is Utilised By Locals As A Food Ingredient. Unfortunately, One Of The Species (Chitala Lopis) Has Already Been Reported Extinct By The IUCN. Nevertheless, Many Publications After That Still Claimed That Chitala Lopis Is Extant. We Study 28 COI Partial Sequences Of Chitala Lopis From NCBI. The Result Showed That Most Of These Sequences Were Not The Actual Chitala Lopis. It Is Probably Either Chitala Hypselonotus Or Chitala Borneensis. This Prediction Is Based On The Unmatch Between The Distribution Of Chitala Lopis COI Sample Origin And The Actual Habitat Of Chitala Lopis, Which Is Endemic To Java Island. However, There Is Also An Article That Reports If Chitala Lopis Is Tried To Be Cultivated. The Misinterpretation Is Perhaps Because Chitala Spp. Is A Complex Species, Which Has Very Similar Morphology. More Study On Molecular Chitala Sp. In Indonesia Is Still Needed To Complete The Unclear Status Of Extinct Chitala Lopi

Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05)

The Effect of Giving Nanoherbal of Biwa (Eriobotrya japonica (Thunb.) Lindl. (Thunb.) Lindl.) Leaf on the Quality and Quantity of Spermatozoa And Microstructure in Diabetes Rat (Rattus norvegicus) Induced by Alloxan

One of causes in decreasing quantity and quality of spermatozoon is disease. Increased blood glucose levels can trigger demage of spermatogenesis in the testes, thereby reducing spermatozoa production. Biwa leaves (Eriobotrya japonica (Thunb.) Lindl.) contain triterpenes which are expected to overcome blood glucose levels and quantity of spermatozoa in the cauda epididymis. To investigate this problem, an experimental test of nano herbal of biwa leaves was carried out in a rat model (Rattus norvegicus) diabet. There are five treatments and six replications, it was found that the best dose of 500 mg/kg body weight could improve spermatogenesis so that the number of rat spermatozoa remained normal. It was concluded that nano herbal of biwa leaves can be used as an alternative drug to maintain fertility as seen from spermatogenesis and the number of spermatozoa. The result is analyzed by ANOVA test and further by Post-Hoc-Test (Duncan). The result show the significant differences (p<0,05) to quantity and quality of spermatozoon of adult white male mouse (Rattus norvegicus)

Histology of Spleen after Induction Nanoherbal Rhodomyrtus tomentosa

The study aim to know to effect of Nanoherbal Rhodomyrtus tomentosa (Haramonting) on spleen histology of Mus musculus. Haramonting in Indonesia as a new source of health-promoting compounds such as dietary fibers, essential fatty acids, and phenolic compounds because there are 19 phenolic compounds including stilbenes and ellagitannins as major components, followed by anthocyanins, flavonols, and gallic acid. Haramonting is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The lungs were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). There were a significant difference (P<0,05) between control group and treatment groups on form of lung cells although no significant in lung weigh

Human Papillomavirus: Detection Method and Infection

The Human Papilloma Virus (HPV) is a small non-enveloped DNA virus, around 8000 bp in size and only humans become its host by infecting skin epithelial tissue, human oral mucosa and anogenital epithelium. Human Papillomavirus is often found in patients and is ranked as the second most malignant disease in women, belonging to the Alphapapillomavirus genus. HPV infection can be identified through the structure of the HPV virus itself and the particles contained therein which initiate the carcinogenic process of its host. The research methods used in this study are literature studies. The literature study method is a series of activities related to the method of collecting library data, reading, recording and managing research materials. The method often used to detect HPV is the polymerase chain reaction (PCR). The viral cell reproduction process is aided by activated proteins E1, E2, E4, E5, E6, and E7, which also delay apoptosis and alter the host cell cycle in various ways to cause cell alterations that result in cancer cells